Investigating CRISPR RNA Biogenesis and Function using RNA-seq
In vitro Co-reconstitution of Cas Protein Complexes
Analysis of CRISPR pre-crRNA Cleavage
Annotation and Classification of CRISPR-Cas Systems
Computational Detection of CRISPR/crRNA Targets
High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium
Spacer-based Macroarrays for CRISPR Genotyping
Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS)
Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR
Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli
Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes
Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus
Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids
Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition
Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl
Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates
Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems
Cas3 Nuclease-Helicase Activity Assays
Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex
Creation and Analysis of a Virome: Using CRISPR Spacers
Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases
Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9
Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).
